Consulta: creatorFacets:"Luo,D.F."
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Data/hora: 19/05/2024 01:29:38

The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
Provedor de dados: 56
Autores: Chen,W.L.; Luo,D.F.; Gao,C.; Ding,Y.; Wang,S.Y.
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear cells (PBMCs) from 119 patients with de novo acute leukemia (AL) and 104 healthy controls, as well as in CD34+ cells from 12 AL patients and 10 healthy donors. A 429-bp fragment from a novel splicing variant of FAMLF was obtained, and a 363-bp consensus sequence was targeted to quantify total FAMLF expression. Kruskal-Wallis, Nemenyi, Spearman's correlation, and Mann-Whitney U-tests were used to analyze...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FAMLF; Gene expression; Leukemia; Real-time polymerase chain reaction; Alternative splicing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700603